Thermo Fisher/赛默飞世尔 | THERMO RNA 纯化试剂盒-20℃ | RNAqueous™-Micro Total RNA Isolation Kit 50 preps

The RNAqueous?-Micro Kit is used for phenol-free total RNA isolation from micro-sized samples (e.g., from <500,000 cultured cells, from few as 10 laser capture microdissected cells, or from ≤10 mg of tissue) using a guanidinium-based lysis/denaturant and glass fiber filter separation technology. DNA-free? DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR. This kit provides:

? Highly concentrated RNA—for increased sensitivity in downstream assays

? Isolation of high-quality RNA from micro-scale samples

? Reagents/protocol for RNA recovery from LCM samples

How it works

Samples are solubilized in a powerful chaotropic solution, and RNA is purified on a glass fiber filter cartridge specifically designed for very small samples. A simple modification of the protocol allows recovery of both large and small RNA species including tRNAs, 5S rRNA, and microRNAs. The RNA is eluted in a minimal volume of 20 μL. Residual genomic DNA is efficiently removed using the DNA-free? system included in the kit. The RNAqueous?-Micro Kit contains enough reagents for 50 RNA isolations of DNA-free RNA.